A general method to quantify ligand-driven oligomerization from fluorescence-based images.
Michael R StonemanGabriel BienerRichard J WardJohn D PedianiDammar BaduAnnie EisIonel PopaGraeme MilliganValerica RaicuPublished in: Nature methods (2019)
Here, we introduce fluorescence intensity fluctuation spectrometry for determining the identity, abundance and stability of protein oligomers. This approach was tested on monomers and oligomers of known sizes and was used to uncover the oligomeric states of the epidermal growth factor receptor and the secretin receptor in the presence and absence of their agonist ligands. This method is fast and is scalable for high-throughput screening of drugs targeting protein-protein interactions.
Keyphrases
- epidermal growth factor receptor
- tyrosine kinase
- advanced non small cell lung cancer
- single molecule
- energy transfer
- deep learning
- high resolution
- cancer therapy
- high intensity
- optical coherence tomography
- convolutional neural network
- protein protein
- amino acid
- small molecule
- wastewater treatment
- mass spectrometry
- solid phase extraction