Long noncoding RNA uc.4 inhibits cell differentiation in heart development by altering DNA methylation.
Qijun ZhangMengwen FengHao ZhangJia XuLi ZhangXuejun WangZijie ChengLingmei QianPublished in: Journal of cellular biochemistry (2018)
In previous studies, we have demonstrated that long noncoding RNA uc.4 may influence the cell differentiation through the TGF-β signaling pathway, suppressed the heart development of zebrafish and resulting cardiac malformation. DNA methylation plays a significant role in the heart development and disordered of DNA methylation may cause disruption of control of gene promoter. In this study, methylated DNA immunoprecipitation was performed to identify the different expression levels of methylation regions. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis were also performed to identify the possible biological process and pathway that uc.4 may join, associated with Rap1 signaling pathway, gonadotropin-releasing hormone signaling pathway, and Calcium signaling pathway. We found that the distribution of differentially methylated regions peaks was mainly located in intergenic and intron regions. Altogether, our result showed that differentially methylated genes are significantly expressed in uc.4-overexpression cells, providing valuable data for further exploration of the role of uc.4 in heart development.
Keyphrases
- dna methylation
- genome wide
- long noncoding rna
- signaling pathway
- induced apoptosis
- pi k akt
- gene expression
- heart failure
- copy number
- epithelial mesenchymal transition
- atrial fibrillation
- genome wide identification
- transcription factor
- poor prognosis
- left ventricular
- endoplasmic reticulum stress
- single molecule
- cell death
- machine learning
- circulating tumor
- artificial intelligence
- oxidative stress
- circulating tumor cells