CRISPR/Cas9-mediated gene knockout and interallelic gene conversion in human induced pluripotent stem cells using non-integrative bacteriophage-chimeric retrovirus-like particles.

Joffrey MiannéAmel NasriChloé Nguyen VanChloé BourguignonMathieu FieldèsEngi AhmedChristine DuthoitNicolas MartinHugues ParrinelloAnaïs LouisAlexandra IchéRégis GayonFlorine SamainLucille LamourouxPascale BouilléArnaud BourdinSaid AssouJohn de Vos

Published in: BMC biology (2022)

Our results highlight the potential of bacteriophage-chimeric retrovirus-like particles to efficiently and safely deliver RNA molecules in hiPSC, and describe for the first time genome engineering by gene conversion in hiPSC. Harnessing this DNA repair mechanism could facilitate the therapeutic correction of human genetic disorders in hiPSC.

Keyphrases

induced pluripotent stem cells
genome wide
dna repair
copy number
crispr cas
endothelial cells
cell therapy
dna damage
genome wide identification
pluripotent stem cells
machine learning
risk assessment
big data
climate change
human health
network analysis
deep learning
nucleic acid