The Effect of Fava Bean ( Vicia faba L.) Protein Ingestion on Myofibrillar Protein Synthesis at Rest and after Resistance Exercise in Healthy, Young Men and Women: A Randomised Control Trial.
Robert W DaviesMarta KoziorArthur E LynchJoseph J BassPhilip J AthertonKenneth SmithPhilip M JakemanPublished in: Nutrients (2022)
The aim of the present study was to evaluate the effect of feeding fava bean ( Vicia faba L.) protein (FBP) on resting and post-exercise myofibrillar fractional synthetic rate (myoFSR). In a parallel, double-blind, randomised control trial, sixteen young, healthy recreationally active adults (age = 25 (5) years, body mass = 70 (15) kg, stature = 1.72 (0.11) m, mean (SD)) ingested 0.33 g·kg -1 FBP ( n = 8) or a negative control (CON, i.e., EAA-free mixture) ( n = 8), immediately after a bout of unilateral knee-extensor resistance exercise. Plasma, saliva, and m. vastus lateralis muscle samples were obtained pre-ingestion and 3 h post-ingestion. MyoFSR was calculated via deuterium labelling of myofibrillar-bound alanine, measured by gas chromatography-pyrolysis-isotope ratio mass spectrometry (GC-Pyr-IRMS). Resistance exercise increased myoFSR ( p = 0.012). However, ingestion of FBP did not evoke an increase in resting (FBP 29 [-5, 63] vs. CON 12 [-25, 49]%, p = 0.409, mean % change [95% CI]) or post-exercise (FBP 78 [33, 123]% vs. CON 58 [9, 107]%, p = 0.732) myoFSR. Ingestion of 0.33 g·kg -1 of FBP does not appear to enhance resting or post-exercise myoFSR in young, healthy, recreationally active adults.
Keyphrases
- high intensity
- gas chromatography
- mass spectrometry
- physical activity
- clinical trial
- double blind
- resistance training
- heart rate
- heart rate variability
- placebo controlled
- study protocol
- tandem mass spectrometry
- randomized controlled trial
- skeletal muscle
- liquid chromatography
- risk assessment
- blood pressure
- high resolution mass spectrometry
- amino acid
- knee osteoarthritis
- high performance liquid chromatography
- gas chromatography mass spectrometry
- ms ms