Structural insights into the EthR-DNA interaction using native mass spectrometry.
Daniel Shiu-Hin ChanWei-Guang SeetohBrendan N McConnellDijana Matak-VinkovićSherine E ThomasVitor MendesMichal BlaszczykAnthony G CoyneTom L BlundellChris AbellPublished in: Chemical communications (Cambridge, England) (2018)
EthR is a transcriptional repressor that increases Mycobacterium tuberculosis resistance to ethionamide. In this study, the EthR-DNA interaction has been investigated by native electrospray-ionization mass spectrometry for the first time. The results show that up to six subunits of EthR are able to bind to its operator.
Keyphrases
- mass spectrometry
- mycobacterium tuberculosis
- circulating tumor
- liquid chromatography
- cell free
- single molecule
- high resolution
- capillary electrophoresis
- gas chromatography
- high performance liquid chromatography
- gene expression
- atomic force microscopy
- nucleic acid
- transcription factor
- pulmonary tuberculosis
- oxidative stress
- circulating tumor cells
- heat shock
- solid phase extraction