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Systematic assessment of tissue dissociation and storage biases in single-cell and single-nucleus RNA-seq workflows.

Elena DenisenkoBelinda B GuoMatthew JonesRui HouLeanne de KockTimo LassmannDaniel PoppeOlivier ClémentRebecca K SimmonsRyan ListerAlistair R R Forrest
Published in: Genome biology (2020)
Systematic comparison of recovered cell types and their transcriptional profiles across the workflows has highlighted protocol-specific biases and thus enables researchers starting single-cell experiments to make an informed choice.
Keyphrases
  • single cell
  • rna seq
  • high throughput
  • randomized controlled trial
  • transcription factor
  • stem cells
  • heat shock
  • cell therapy