Systematic assessment of tissue dissociation and storage biases in single-cell and single-nucleus RNA-seq workflows.
Elena DenisenkoBelinda B GuoMatthew JonesRui HouLeanne de KockTimo LassmannDaniel PoppeOlivier ClémentRebecca K SimmonsRyan ListerAlistair R R ForrestPublished in: Genome biology (2020)
Systematic comparison of recovered cell types and their transcriptional profiles across the workflows has highlighted protocol-specific biases and thus enables researchers starting single-cell experiments to make an informed choice.