Soybean Oil-Based 3D Printed Mesh Designed for Guided Bone Regeneration (GBR) in Oral Surgery.

This study aims to obtain a cyto-compatible 3D printable bio-resin for the manufacturing of meshes designed from acquired real patients' bone defect, to be used in future for Guided Bone Regeneration (GBR) achieving the goal of personalized medicine decreasing surgical, recovery time and patient discomfort. To this purpose, a biobased, biocompatible and photo-curable resin made of acrylated epoxidized soybean oil (AESO) diluted with soybean oil (SO) was developed and 3D printed using a commercial digital light processing (DLP) 3D printer. 3D printed samples have shown good thermal properties allowing for thermally-based sterilization process, and mechanical properties typical of crosslinked natural oils (i.e., E = 12 MPa, UTS = 1.5 MPa) suitable for the GBR application in the oral surgery. The AESO-SO bio-resin has proved to be cytocompatible allowing for fibroblast cells proliferation (viability at 72 h > 97%), without inducing severe inflammatory response when co-cultured with macrophages, as demonstrated by cytokine antibody arrays, that is anyway resolved in the first 24 hours. Moreover accelerated degradation tests proved that the bio-resin is biodegradable in hydrolytic environments. This article is protected by copyright. All rights reserved.