Embryonic growth retardation and altered expression of IGF-II is reciprocal induced by phytocompounds during early gestation in mice.
Khamhee WangsaKrishnakshi MisraUpasa GowalaIndira SarmaPurba Jyoti SaikiaHirendra Nath SarmaPublished in: Growth factors (Chur, Switzerland) (2022)
Methanolic crude extract of Scoparia dulcis (CESD) was orally administered to female mice during the early gestation (day 4-day 8) at a dose of 500 mg/kg/day. It induces embryo resorption and morphological changes of fetal maternal tissue. Histomorphology was studied by routine hematoxylin eosin stain. In situ immunofluorescence localization of IGF-II using Texas red showed an ordered expression of the growth factor in the maternal decidual cells, trophoblast cells and the embryo. Western blot analysis showed a gradual increase of IGF-II from D4 to D8 of control females. In contrast, the CESD-treated females showed resorption of embryo on D8 with disorganized in situ expression and lowered IGF-II in fetal maternal tissue. The phytocompounds present in the CESD could modulate either the ER or IGF-II receptors causing reduced IGF-II expression in the target tissues which lead to the failure of embryonic growth during periimplantation.
Keyphrases
- binding protein
- poor prognosis
- growth factor
- pregnancy outcomes
- induced apoptosis
- pi k akt
- growth hormone
- preterm infants
- magnetic resonance
- gene expression
- pregnant women
- oxidative stress
- signaling pathway
- endoplasmic reticulum stress
- computed tomography
- south africa
- metabolic syndrome
- cell death
- estrogen receptor
- contrast enhanced