A Magnetic Iron Oxide/Polydopamine Coating Can Improve Osteogenesis of 3D-Printed Porous Titanium Scaffolds with a Static Magnetic Field by Upregulating the TGFβ-Smads Pathway.
Zhenfei HuangYu HeXiao ChangJieying LiuLingjia YuYuanhao WuYaqian LiJingjing TianLin KangDi WuHai WangZhihong WuGuixing QiuPublished in: Advanced healthcare materials (2020)
3D-printed porous titanium-aluminum-vanadium (Ti6Al4V, pTi) scaffolds offer surgeons a good option for the reconstruction of large bone defects, especially at the load-bearing sites. However, poor osteogenesis limits its application in clinic. In this study, a new magnetic coating is successfully fabricated by codepositing of Fe3 O4 nanoparticles and polydopamine (PDA) on the surface of 3D-printed pTi scaffolds, which enhances cell attachment, proliferation, and osteogenic differentiation of hBMSCs in vitro and new bone formation of rabbit femoral bone defects in vivo with/without a static magnetic field (SMF). Furthermore, through proteomic analysis, the enhanced osteogenic effect of the magnetic Fe3 O4 /PDA coating with the SMF is found to be related to upregulate the TGFβ-Smads signaling pathway. Therefore, this work provides a simple protocol to improve the osteogenesis of 3D-printed porous pTi scaffolds, which will help their application in clinic.
Keyphrases
- tissue engineering
- transforming growth factor
- signaling pathway
- bone regeneration
- molecularly imprinted
- epithelial mesenchymal transition
- primary care
- bone mineral density
- iron oxide
- metal organic framework
- mesenchymal stem cells
- randomized controlled trial
- soft tissue
- single cell
- cell therapy
- postmenopausal women
- mass spectrometry
- induced apoptosis
- pi k akt
- body composition
- liquid chromatography
- thoracic surgery
- tandem mass spectrometry