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Living Cell Nanoporation and Exosomal RNA Analysis Platform for Real-Time Assessment of Cellular Therapies.

Yan ShengZena HuangTenghua ZhangFeiyang QianYuqing ZhuZaizai DongQingyang ZhangQi LeiFanhui KongYuqiong WangMaddie WaldenStefan WuttkeLingqian ChangWei ZhuJiaming Hu
Published in: Journal of the American Chemical Society (2022)
Efficient transfection of therapeutic agents and timely potency testing are two key factors hindering the development of cellular therapy. Here we present a cellular-nanoporation and exosome assessment device, a quantitative platform for nanochannel-based cell electroporation and exosome-based in situ RNA expression analysis. In its application to transfection of anti-miRNAs and/or chemotherapeutics into cells, we have systematically described the differences in RNA expression in secreted exosomes and assessed cellular therapies in real time.
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