The mitochondrial genome (mitogenome) of Ornithomya biloba (Dufour 1827) was first sequenced and annotated in this study as the first representative of the genus Ornithomya . The complete mitogenome is 18,654 bp in length and contains 37 genes (13 protein-coding genes (PCGs), 22 tRNA genes, two rRNA genes, and control region). The phylogenetic analysis based on 13 PCGs in IQ-TREE supports the monophyly of Hippoboscidae, which was a sister group of Streblidae. Families Hippoboscidae and Streblidae formed the monophyletic Hippoboscoidea clade.