Portable Mid-Infrared Spectroscopy Combined with Chemometrics to Diagnose Fibromyalgia and Other Rheumatologic Syndromes Using Rapid Volumetric Absorptive Microsampling.
Shreya Madhav NuguriKevin V HackshawSilvia de Lamo CastellviHaona BaoSiyu YaoRija AzizScott SelingerZhanna MikulikLianbo YuMichelle M Osuna-DiazKatherine R SebastianMaria Monica GiustiLuis Rodriguez-SaonaPublished in: Molecules (Basel, Switzerland) (2024)
The diagnostic criteria for fibromyalgia (FM) have relied heavily on subjective reports of experienced symptoms coupled with examination-based evidence of diffuse tenderness due to the lack of reliable biomarkers. Rheumatic disorders that are common causes of chronic pain such as rheumatoid arthritis, systemic lupus erythematosus, osteoarthritis, and chronic low back pain are frequently found to be comorbid with FM. As a result, this can make the diagnosis of FM more challenging. We aim to develop a reliable classification algorithm using unique spectral profiles of portable FT-MIR that can be used as a real-time point-of-care device for the screening of FM. A novel volumetric absorptive microsampling (VAMS) technique ensured sample volume accuracies and minimized the variation introduced due to hematocrit-based bias. Blood samples from 337 subjects with different disorders (179 FM, 158 non-FM) collected with VAMS were analyzed. A semi-permeable membrane filtration approach was used to extract the blood samples, and spectral data were collected using a portable FT-MIR spectrometer. The OPLS-DA algorithm enabled the classification of the spectra into their corresponding classes with 84% accuracy, 83% sensitivity, and 85% specificity. The OPLS-DA regression plot indicated that spectral regions associated with amide bands and amino acids were responsible for discrimination patterns and can be potentially used as spectral biomarkers to differentiate FM and other rheumatic diseases.
Keyphrases
- rheumatoid arthritis
- machine learning
- optical coherence tomography
- deep learning
- chronic pain
- systemic lupus erythematosus
- cell proliferation
- disease activity
- long non coding rna
- long noncoding rna
- dual energy
- amino acid
- high resolution
- big data
- emergency department
- magnetic resonance imaging
- artificial intelligence
- magnetic resonance
- computed tomography
- neural network
- low grade
- systemic sclerosis
- knee osteoarthritis
- physical activity
- molecular dynamics
- density functional theory
- drug induced
- idiopathic pulmonary fibrosis