Epidemiological features of nosocomial Klebsiella pneumoniae: virulence and resistance determinants.
Mai M ZaferMaha M El BastawisieMona WassefAmira Fa HusseinMohammed A RamadanPublished in: Future microbiology (2021)
Aim: The authors aimed to examine antibiotic resistance genes and representative virulence determinants among 100 Klebsiella pneumoniae isolates with an emphasis on capsular serotypes and clonality of some of the isolates. Methods: PCR amplification of (rmpA, rmpA2, iutA, iroN and IncHI1B plasmid) and (NDM, OXA-48, KPC, CTX-M-15, VIM, IMP, SPM) was conducted. Wzi sequencing and multilocus sequence typing (MLST) were performed. Results: K2 was the only detected serotype in the authors' collection. RMPA2 was the most common capsule-associated virulence gene detected. All studied isolates harbored OXA-48-like (100%) and NDM (43%) (n = 43). ST147 was the most common sequence type. Conclusion: This work provides insight into the evolution of the coexistence of virulence and resistance genes in a tertiary healthcare setting in Cairo, Egypt.
Keyphrases
- klebsiella pneumoniae
- escherichia coli
- biofilm formation
- pseudomonas aeruginosa
- antibiotic resistance genes
- healthcare
- genetic diversity
- multidrug resistant
- staphylococcus aureus
- antimicrobial resistance
- genome wide
- microbial community
- wastewater treatment
- genome wide identification
- cross sectional
- dna methylation
- anaerobic digestion
- copy number
- social media
- solid state