Mapping the immunogenic landscape of near-native HIV-1 envelope trimers in non-human primates.
Christopher A CottrellJelle van SchootenCharles A BowmanMeng YuanDavid OyenMia ShinRobert MorpurgoPatricia van der WoudeMariëlle van BreemenJonathan L TorreRaj PatelJustin GrossLeigh M SewallJeffrey CoppsGabriel OzorowskiBartek NogalDevin SokEva G RakaszCelia C LaBrancheVladimir VigdorovichScott ChristleyDiane G CarnathanD Noah SatherDavid C MontefioriGuido SilvestriDennis R BurtonJohn P MooreIan A WilsonRogier W SandersAndrew B WardMarit J VAN GilsPublished in: PLoS pathogens (2020)
The induction of broad and potent immunity by vaccines is the key focus of research efforts aimed at protecting against HIV-1 infection. Soluble native-like HIV-1 envelope glycoproteins have shown promise as vaccine candidates as they can induce potent autologous neutralizing responses in rabbits and non-human primates. In this study, monoclonal antibodies were isolated and characterized from rhesus macaques immunized with the BG505 SOSIP.664 trimer to better understand vaccine-induced antibody responses. Our studies reveal a diverse landscape of antibodies recognizing immunodominant strain-specific epitopes and non-neutralizing neo-epitopes. Additionally, we isolated a subset of mAbs against an epitope cluster at the gp120-gp41 interface that recognize the highly conserved fusion peptide and the glycan at position 88 and have characteristics akin to several human-derived broadly neutralizing antibodies.
Keyphrases
- endothelial cells
- antiretroviral therapy
- hiv infected
- induced pluripotent stem cells
- human immunodeficiency virus
- hiv positive
- hepatitis c virus
- hiv aids
- dengue virus
- single cell
- pluripotent stem cells
- high glucose
- hiv testing
- high resolution
- south africa
- dna methylation
- machine learning
- platelet rich plasma
- quality improvement
- cell therapy
- aedes aegypti