Haploid genetic screens identify SPRING/C12ORF49 as a determinant of SREBP signaling and cholesterol metabolism.
Anke LoreggerMatthijs RaabenJoppe NieuwenhuisJosephine M E TanLucas T JaeLisa G van den HengelSebastian HendrixMarlene van den BergSaskia ScheijJi-Ying SongIvo Johan HuijbersLona J KroeseRoelof OttenhoffMichel van WeeghelBart van de SluisThijn BrummelkampNoam ZelcerPublished in: Nature communications (2020)
The sterol-regulatory element binding proteins (SREBP) are central transcriptional regulators of lipid metabolism. Using haploid genetic screens we identify the SREBP Regulating Gene (SPRING/C12ORF49) as a determinant of the SREBP pathway. SPRING is a glycosylated Golgi-resident membrane protein and its ablation in Hap1 cells, Hepa1-6 hepatoma cells, and primary murine hepatocytes reduces SREBP signaling. In mice, Spring deletion is embryonic lethal yet silencing of hepatic Spring expression also attenuates the SREBP response. Mechanistically, attenuated SREBP signaling in SPRINGKO cells results from reduced SREBP cleavage-activating protein (SCAP) and its mislocalization to the Golgi irrespective of the cellular sterol status. Consistent with limited functional SCAP in SPRINGKO cells, reintroducing SCAP restores SREBP-dependent signaling and function. Moreover, in line with the role of SREBP in tumor growth, a wide range of tumor cell lines display dependency on SPRING expression. In conclusion, we identify SPRING as a previously unrecognized modulator of SREBP signaling.
Keyphrases
- induced apoptosis
- cell cycle arrest
- genome wide
- poor prognosis
- transcription factor
- gene expression
- endoplasmic reticulum stress
- binding protein
- high throughput
- oxidative stress
- metabolic syndrome
- patient safety
- dna methylation
- skeletal muscle
- long non coding rna
- quality improvement
- protein protein
- genome wide identification