Bone Targeted Delivery of SDF-1 via Alendronate Functionalized Nanoparticles in Guiding Stem Cell Migration.
Qingchang ChenChuping ZhengYanqun LiShaoquan BianHaobo PanXiaoli ZhaoWilliam W LuPublished in: ACS applied materials & interfaces (2018)
Stem cells are well-known for their great capacity for tissue regeneration. This provides a promising source for cell-based therapies in treating various bone degenerative disorders. However, the major hurdles for their application in transplantation are the poor bone marrow homing and engraftment efficiencies. Stromal cell-derived factor 1 (SDF-1) has been identified as a major stem cell homing factor. With the aims of bone targeted SDF-1 delivery and regulating MSCs migration, alendronate modified liposomal nanoparticles (Aln-Lipo) carrying SDF-1 gene were developed in this study. Alendronate modification significantly increased the mineral binding affinity of liposomes, and facilitated the gene delivery to osteoblastic cells. Up-regulated SDF-1 expression in osteoblasts triggered MSCs migration. Systemic infusion of Aln-Lipo-SDF-1 with fluorescence labeling in mice showed the accumulation in osseous tissue by biophotonic imaging. Corresponding to the delivered SDF-1, the transplanted GFP+ MSCs were attracted to bone marrow and contributed to bone regeneration. This study may provide a useful technique in regulating stem cell migration.
Keyphrases
- stem cells
- cell migration
- bone marrow
- mesenchymal stem cells
- bone regeneration
- cell therapy
- bone mineral density
- umbilical cord
- high resolution
- induced apoptosis
- poor prognosis
- drug delivery
- type diabetes
- single cell
- dna methylation
- postmenopausal women
- metabolic syndrome
- copy number
- insulin resistance
- cell proliferation
- binding protein
- cell death
- cell cycle arrest
- signaling pathway
- mass spectrometry
- pi k akt
- cord blood