The lineage relationship of clonally-related cells offers important insights into the ontogeny and cytoarchitecture of the brain in health and disease. Here, we provide a protocol to concurrently assess cell lineage relationship and cell-type identity among clonally-related cells in situ. We first describe the preparation and screening of acute brain slices containing clonally-related cells labeled using mosaic analysis with double markers (MADM). We then outline steps to collect RNA from individual cells for downstream applications and cell-type identification using RNA sequencing. For complete details on the use and execution of this protocol, please refer to Cheung et al. 1 .
Keyphrases
- induced apoptosis
- cell cycle arrest
- single cell
- healthcare
- randomized controlled trial
- endoplasmic reticulum stress
- oxidative stress
- high resolution
- white matter
- stem cells
- functional connectivity
- resting state
- intensive care unit
- computed tomography
- social media
- cell proliferation
- bone marrow
- health information
- hepatitis b virus
- extracorporeal membrane oxygenation
- liquid chromatography
- aortic dissection
- data analysis