Effects of Motilin Receptor Agonists and Ghrelin in Human motilin receptor Transgenic Mice.
Tomoe KawamuraBunzo MatsuuraTeruki MiyakeMasanori AbeYoshiou IkedaYoichi HiasaPublished in: International journal of molecular sciences (2019)
Gastrointestinal motility is regulated by neural factors and humoral factors. Both motilin and ghrelin improve gastrointestinal motility, but many issues remain unclear. We prepared human motilin receptor transgenic (Tg) mice and performed experiments evaluating the effects of motilin, erythromycin (EM), and ghrelin. EM and ghrelin promoted gastric emptying (GE) when administered either peripherally or centrally to Tg mice. Atropine (a muscarinic receptor antagonist) counteracted GE induced by centrally administered EM, but not that induced by peripherally administered EM. The administration of EM in this model promoted the effect of mosapride (a selective serotonin 5-hydroxytryptamine 4 (5-HT4) receptor agonist), and improved loperamide (a μ-opioid receptor agonist)-induced gastroparesis. The level of acyl-ghrelin was significantly attenuated by EM administration. Thus, we have established an animal model appropriate for the evaluation of motilin receptor agonists. These data and the model are expected to facilitate the identification of novel compounds with clinical potential for relieving symptoms of dyspepsia and gastroparesis.
Keyphrases
- endothelial cells
- growth hormone
- immune response
- high glucose
- chronic pain
- induced pluripotent stem cells
- high fat diet induced
- biofilm formation
- electronic health record
- type diabetes
- escherichia coli
- metabolic syndrome
- depressive symptoms
- machine learning
- adipose tissue
- fatty acid
- helicobacter pylori
- sleep quality
- deep learning