Single cell sequencing identifies clonally expanded synovial CD4 + T PH cells expressing GPR56 in rheumatoid arthritis.
Alexandra ArgyriouMarc H WadsworthAdrian LendvaiStephen M ChristensenAase H HensvoldChristina GerstnerAnnika van VollenhovenKellie M KravarikAaron WinklerVivianne MalmströmKarine CheminPublished in: Nature communications (2022)
Rheumatoid arthritis (RA) is an autoimmune disease affecting synovial joints where different CD4 + T cell subsets may contribute to pathology. Here, we perform single cell sequencing on synovial CD4 + T cells from anti-citrullinated protein antibodies (ACPA)+ and ACPA- RA patients and identify two peripheral helper T cell (T PH ) states and a cytotoxic CD4 + T cell subset. We show that the adhesion G-protein coupled receptor 56 (GPR56) delineates synovial CXCL13 high T PH CD4 + T cells expressing LAG-3 and the tissue-resident memory receptors CXCR6 and CD69. In ACPA- SF, T PH cells display lower levels of GPR56 and LAG-3. Further, most expanded T cell clones in the joint are within CXCL13 high T PH CD4 + T cells. Finally, RNA-velocity analyses suggest a common differentiation pathway between the two T PH clusters and effector CD4 + T cells. Our study provides comprehensive immunoprofiling of the synovial CD4 + T cell subsets in ACPA+ and ACPA- RA.
Keyphrases
- rheumatoid arthritis
- single cell
- disease activity
- induced apoptosis
- rna seq
- ankylosing spondylitis
- cell cycle arrest
- regulatory t cells
- interstitial lung disease
- end stage renal disease
- dendritic cells
- peripheral blood
- fatty acid
- newly diagnosed
- ejection fraction
- genome wide
- gene expression
- multiple sclerosis
- prognostic factors
- nk cells
- cystic fibrosis
- signaling pathway