Combined matrix-assisted laser desorption/ionisation-mass spectrometry imaging with liquid chromatography-tandem mass spectrometry for observing spatial distribution of lipids in whole Caenorhabditis elegans.
Michiel VandenboschErika R Amstalden van HoveRonny MohrenIsabeau VermeulenHenry B DijkmanRon M A HeerenPim E G LeonardsSamantha HughesPublished in: Rapid communications in mass spectrometry : RCM (2024)
This optimised sample preparation technique enabled (un)targeted analysis of spatially distributed lipids within individual nematodes. The possibility to detect other biomolecules using this method thus laid the basis for prospective preclinical and toxicological studies on C. elegans.
Keyphrases
- liquid chromatography tandem mass spectrometry
- mass spectrometry
- simultaneous determination
- high resolution
- solid phase extraction
- liquid chromatography
- high performance liquid chromatography
- ms ms
- molecularly imprinted
- fatty acid
- tandem mass spectrometry
- gas chromatography
- cancer therapy
- case control
- high speed
- cell therapy
- capillary electrophoresis
- bone marrow
- neural network