Emulsion Cross-Linking Technique for Human Fibroblast Encapsulation.
Watcharaphong ChaemsawangWeerapong PrasongcheanKonstantinos I PapadopoulosSuchada SukrongW John KaoPhanphen WattanaarsakitPublished in: International journal of biomaterials (2018)
Microencapsulation with biodegradable polymers has potential application in drug and cell delivery systems and is currently used in probiotic delivery. In the present study, microcapsules of human fibroblast cells (CRL2522) were prepared by emulsion cross-linking technique. Tween 80 surfactant at a 2% concentration through phase inversion resulted in the most efficient and stable size, morphology, and the cells survival at least 50% on day 14. Emulsion cross-linking microcapsule preparation resulted in smaller and possibly more diverse particles that can be developed clinically to deliver encapsulated mammalian cells for future disease treatments.
Keyphrases
- induced apoptosis
- endothelial cells
- cell cycle arrest
- pluripotent stem cells
- cell death
- endoplasmic reticulum stress
- single cell
- emergency department
- cell therapy
- signaling pathway
- oxidative stress
- risk assessment
- stem cells
- mass spectrometry
- magnetic resonance
- computed tomography
- climate change
- pi k akt
- adverse drug