Vibrational spectroscopy techniques can provide comprehensive information on lipogenesis in dimorphic and oleaginous fungi at the levels of the bulk of cells, single cells and single lipid droplets. Unicellular spectra showed that various cell forms of M. circinelloides differs in the total lipid content and profile of the accumulated lipids, where yeast-like cells are the fatty ones and, therefore, could be considered as preferable cell form for producing lipid-rich biomass. Spectra of single lipid droplets showed an indication of possible droplet-to-droplet and within-droplet heterogeneity.
Keyphrases
- single cell
- fatty acid
- induced apoptosis
- high throughput
- cell cycle arrest
- density functional theory
- cell therapy
- stem cells
- endoplasmic reticulum stress
- type diabetes
- molecular docking
- signaling pathway
- cell death
- oxidative stress
- molecular dynamics simulations
- wastewater treatment
- mass spectrometry
- mesenchymal stem cells
- saccharomyces cerevisiae
- molecular dynamics