Quantitative phase measurements of human cell nuclei using X-ray ptychography.
Jorg SchwenkeMohammed YusufLaura A ShemiltUlrich WagnerAtiqa SajidGraeme R MorrisonFucai ZhangAaron D ParsonsChristoph RauIan K RobinsonPublished in: Journal of synchrotron radiation (2021)
The human cell nucleus serves as an important organelle holding the genetic blueprint for life. In this work, X-ray ptychography was applied to assess the masses of human cell nuclei using its unique phase shift information. Measurements were carried out at the I13-1 beamline at the Diamond Light Source that has extremely large transverse coherence properties. The ptychographic diffractive imaging approach allowed imaging of large structures that gave quantitative measurements of the phase shift in 2D projections. In this paper a modified ptychography algorithm that improves the quality of the reconstruction for weak scattering samples is presented. The application of this approach to calculate the mass of several human nuclei is also demonstrated.
Keyphrases
- high resolution
- endothelial cells
- induced pluripotent stem cells
- single cell
- pluripotent stem cells
- machine learning
- computed tomography
- stem cells
- magnetic resonance
- healthcare
- magnetic resonance imaging
- deep learning
- gene expression
- bone marrow
- fluorescence imaging
- mesenchymal stem cells
- photodynamic therapy
- dual energy
- quality improvement