Cell-based assays for the detection of MOG antibodies: a comparative study.

Matteo GastaldiSilvia ScaranzinSven JariusBrigitte WildemanElisabetta ZardiniGiulia MallucciEleonora RigoniElisa VegezziThomas FoiadelliSalvatore SavastaPaola BanfiMaurizio VersinoLuana BenedettiGiovanni NoviMargherita Maria MancardiThea GiacominiPietro AnnovazziDamiano BaronciniDiana FerraroVito LampasonaMarkus ReindlPatrick WatersDiego Franciotta

Published in: Journal of neurology (2020)

LCBA-IgG1 showed the highest specificity but can miss MOG-IgG2 reactivities, whose meaning warrants further investigations. Titration of samples tested with LCBA-IgGH+L/ IgGFcγ is important for meaningful interpretation of the results. In the subgroup analysis, LCBA-IgGFcγ yielded the highest accuracy, and FCBA-IgGFcγ good specificity, but it was at risk of false-negative results.

Keyphrases

single cell
structural basis
loop mediated isothermal amplification
stem cells
randomized controlled trial
mesenchymal stem cells
phase iii
open label
quantum dots