Cobalt Chromium Molybdenum Surface Modifications Alter the Osteogenic Differentiation Potential of Human Mesenchymal Stem Cells.
Birgit LohbergerNicole EckDietmar GlaenzerHelga C LichteneggerLeon PloszczanskiAndreas LeithnerPublished in: Materials (Basel, Switzerland) (2020)
Surface roughness on orthopedic implant materials has been shown to be highly influential on the behavior of osteogenic cells. Mesenchymal stem and progenitor cells (MSPCs) migrate to the interface, adhere, proliferate, and differentiate into osteoblasts, which subsequently form bone matrix. Modifications of the implant surfaces should accelerate this process and improve biocompatibility. In this study, five surface topographies on cobalt chromium molybdenum (CoCrMo) were engineered to examine the influence on MSPCs. Scanning electron microscopy revealed significant differences in the morphology of untreated CoCrMo discs in comparison with CoCrMo with a titanium nitride (TiN) coating, polished and porous coated CoCrMo surfaces, and CoCrMo with a pure titanium (cpTi) coating. Elemental analysis was performed using energy-dispersive X-ray spectroscopy (EDX). Human primary MSPCs were expanded from tissue samples of spongiosa bone and characterized according to the criteria of the International Society for Cellular Therapy. The characteristic phenotype of MSPC was confirmed by flow cytometry and multilineage differentiation. Alcaline phosphatase and osteopontin expression increased significantly in all groups about 5-fold and 10-fold, respectively, in comparison to the undifferentiated controls. The porous coated surface showed a reduced expression of osteogenic markers. Due to the osteogenic differentiation, the expression of integrin α5β1, which is particularly important for cell-material contact, increased 4-7-fold. In the dynamic process of bone biology, MSPCs cultured and differentiated on cpTi, showed significant upregulation of IL6 and leptin.
Keyphrases
- mesenchymal stem cells
- poor prognosis
- bone marrow
- electron microscopy
- endothelial cells
- soft tissue
- umbilical cord
- bone mineral density
- flow cytometry
- cell therapy
- high resolution
- metal organic framework
- binding protein
- induced apoptosis
- long non coding rna
- stem cells
- single cell
- magnetic resonance
- tissue engineering
- oxidative stress
- computed tomography
- mass spectrometry
- magnetic resonance imaging
- risk assessment
- body composition
- pseudomonas aeruginosa
- postmenopausal women
- staphylococcus aureus
- signaling pathway
- cell cycle arrest
- single molecule
- protein kinase
- cell proliferation
- bone regeneration
- smoking cessation
- human health
- cell death
- solid phase extraction
- pi k akt
- tandem mass spectrometry
- climate change
- gas chromatography mass spectrometry
- visible light
- perovskite solar cells