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Single-molecule study on conformational dynamics of M.HhaI.

Shanshan HeChen YangSijia PengChunlai ChenXin Sheng Zhao
Published in: RSC advances (2019)
We found that apo DNA methyltransferase M.HhaI under the physiological salt concentration does not possess the structure characterized by X-ray crystallography; instead, it interchanges between prefolded and unfolded states. Only after binding to the substrate, it transforms into a crystal-structure-like state. Flipping rates of its catalytic loop were directly measured.
Keyphrases
  • single molecule
  • crystal structure
  • living cells
  • atomic force microscopy
  • endoplasmic reticulum stress
  • computed tomography
  • molecular dynamics
  • dual energy
  • molecular dynamics simulations
  • structural basis