Efficient CRISPR/Cas9 genome editing with Citrus embryogenic cell cultures.

Manjul DuttZhonglin MouXudong ZhangSameena E TanwirJude W Grosser

Published in: BMC biotechnology (2020)

Both the pC-PDS1 and pC-PDS2 could successfully edit the citrus embryogenic cell cultures. However, the editing efficiency was dependent on the gRNA, confirming that the selection of a proper gRNA is essential for successful genome editing using the CRISPR/Cas9 technique. Also, utilization of embryogenic cell cultures offers another option for successful genome editing in citrus.

Keyphrases

crispr cas
genome editing
single cell
cell therapy
mesenchymal stem cells