Single-cell technologies to decipher cardiovascular diseases.
Wesley Tyler AbplanalpNathan R TuckerStefanie DimmelerPublished in: European heart journal (2022)
Cardiovascular disease remains the leading cause of death worldwide. A deeper understanding of the multicellular composition and molecular processes may help to identify novel therapeutic strategies. Single-cell technologies such as single-cell or single-nuclei RNA sequencing provide expression profiles of individual cells and allow for dissection of heterogeneity in tissue during health and disease. This review will summarize (i) how these novel technologies have become critical for delineating mechanistic drivers of cardiovascular disease, particularly, in humans and (ii) how they might serve as diagnostic tools for risk stratification or individualized therapy. The review will further discuss technical pitfalls and provide an overview of publicly available human and mouse data sets that can be used as a resource for research.
Keyphrases
- single cell
- cardiovascular disease
- rna seq
- high throughput
- induced apoptosis
- endothelial cells
- type diabetes
- public health
- healthcare
- cardiovascular events
- cardiovascular risk factors
- mental health
- electronic health record
- cell cycle arrest
- stem cells
- oxidative stress
- health information
- big data
- machine learning
- cell death
- mesenchymal stem cells
- cell proliferation
- bone marrow
- smoking cessation
- pluripotent stem cells