Benchmarking ultra-high molecular weight DNA preservation methods for long-read and long-range sequencing.
Hollis A DahnJacquelyn MountcastleJennifer R BalaccoSylke WinklerIliana BistaAnthony D SchmittOlga Vinnere PetterssonGiulio FormentiKaren OliverMichelle SmithWenhua TanAnne KrausStephen MacLisa M KomoroskeTanya M LamaAndrew J CrawfordRobert W MurphySamara BrownAlan F ScottPhillip A MorinErich D JarvisOlivier FedrigoPublished in: GigaScience (2022)
We provide sample preservation guidelines that ensure sufficient DNA integrity and amount required for use with long-read and long-range sequencing technologies across vertebrates. Our best practices generated the uHMW DNA needed for the high-quality reference genomes for phase 1 of the Vertebrate Genomes Project, whose ultimate mission is to generate chromosome-level reference genome assemblies of all ∼70,000 extant vertebrate species.