Endothelial NOX5 overexpression induces changes in the cardiac gene profile: potential impact in myocardial infarction?
Adriana CortésJavier MarquésÁlvaro PejenauteElena AinzúaEduardo AnsorenaGloria AbizandaFelipe PrósperCarlos de MiguelGuillermo ZalbaPublished in: Journal of physiology and biochemistry (2023)
Cardiovascular diseases and the ischemic heart disease specifically constitute the main cause of death worldwide. The ischemic heart disease may lead to myocardial infarction, which in turn triggers numerous mechanisms and pathways involved in cardiac repair and remodeling. Our goal in the present study was to characterize the effect of the NADPH oxidase 5 (NOX5) endothelial expression in healthy and infarcted knock-in mice on diverse signaling pathways. The mechanisms studied in the heart of mice were the redox pathway, metalloproteinases and collagen pathway, signaling factors such as NFκB, AKT or Bcl-2, and adhesion molecules among others. Recent studies support that NOX5 expression in animal models can modify the environment and predisposes organ response to harmful stimuli prior to pathological processes. We found many alterations in the mRNA expression of components involved in cardiac fibrosis as collagen type I or TGF-β and in key players of cardiac apoptosis such as AKT, Bcl-2, or p53. In the heart of NOX5-expressing mice after chronic myocardial infarction, gene alterations were predominant in the redox pathway (NOX2, NOX4, p22phox, or SOD1), but we also found alterations in VCAM-1 and β-MHC expression. Our results suggest that NOX5 endothelial expression in mice preconditions the heart, and we propose that NOX5 has a cardioprotective role. The correlation studies performed between echocardiographic parameters and cardiac mRNA expression supported NOX5 protective action.
Keyphrases
- left ventricular
- reactive oxygen species
- poor prognosis
- heart failure
- signaling pathway
- high fat diet induced
- cell proliferation
- cardiovascular disease
- endothelial cells
- copy number
- atrial fibrillation
- type diabetes
- mitral valve
- left atrial
- endoplasmic reticulum stress
- gene expression
- escherichia coli
- pulmonary hypertension
- transcription factor
- risk assessment
- coronary artery disease
- genome wide
- pi k akt
- metabolic syndrome
- pseudomonas aeruginosa
- cystic fibrosis
- living cells
- sensitive detection
- case control
- ejection fraction
- candida albicans