Hericium Erinaceus Prevents DEHP-Induced Mitochondrial Dysfunction and Apoptosis in PC12 Cells.
Ines AmaraMaria ScutoAgata ZappalàMaria Laura OntarioAntonio PetraliaSalwa Abid-EssefiLuigi MaiolinoAnna SignorileAngela Trovato SalinaroVittorio CalabresePublished in: International journal of molecular sciences (2020)
Hericium Erinaceus (HE) is a medicinal plant known to possess anticarcinogenic, antibiotic, and antioxidant activities. It has been shown to have a protective effect against ischemia-injury-induced neuronal cell death in rats. As an extending study, here we examined in pheochromocytoma 12 (PC12) cells, whether HE could exert a protective effect against oxidative stress and apoptosis induced by di(2-ethylhexyl)phthalate (DEHP), a plasticizer known to cause neurotoxicity. We demonstrated that pretreatment with HE significantly attenuated DEHP induced cell death. This protective effect may be attributed to its ability to reduce intracellular reactive oxygen species levels, preserving the activity of respiratory complexes and stabilizing the mitochondrial membrane potential. Additionally, HE pretreatment significantly modulated Nrf2 and Nrf2-dependent vitagenes expression, preventing the increase of pro-apoptotic and the decrease of anti-apoptotic markers. Collectively, our data provide evidence of new preventive nutritional strategy using HE against DEHP-induced apoptosis in PC12 cells.
Keyphrases
- oxidative stress
- cell death
- diabetic rats
- induced apoptosis
- cell cycle arrest
- endoplasmic reticulum stress
- high glucose
- ischemia reperfusion injury
- reactive oxygen species
- dna damage
- drug induced
- poor prognosis
- anti inflammatory
- pseudomonas aeruginosa
- mouse model
- heat shock
- signaling pathway
- risk assessment
- long non coding rna
- big data
- cystic fibrosis