N-Acetylcysteine (NAC) Ameliorates Lipid-Related Metabolic Dysfunction in Bone Marrow Stromal Cells-Derived Adipocytes.
Marco RaffaeleIgnazio BarbagalloMaria LicariGiuseppe CarotaGiuseppe SferrazzoMariarita SpampinatoSorrenti ValeriaLuca VanellaPublished in: Evidence-based complementary and alternative medicine : eCAM (2018)
Recent experimental data suggest that fatty acids and lipotoxicity could play a role in the initiation and evolution of metabolic bone diseases such as osteoporosis. A functional bone marrow adipose tissue (BMAT) may provide support to surrounding cells and tissues or may serve as a lipid reservoir that protects skeletal osteoblasts from lipotoxicity. The present study examined the effect of N-acetylcysteine (NAC), a powerful antioxidant and precursor of glutathione, commonly used to treat chronic obstructive pulmonary disease, on triglycerides accumulation in bone marrow stromal cells-derived adipocytes. Quantification of Oil Red O stained cells showed that lipid droplets decreased following NAC treatment. Additionally, exposure of bone marrow stromal cells (HS-5) to NAC increased adiponectin, PPARγ, HO-1, and SIRT-1 and increased beta-oxidation markers such as PPARα and PPARδ mRNA levels. As there is now substantial interest in alternative medicine, the observed therapeutic value of NAC should be taken into consideration in diabetic patients.
Keyphrases
- bone marrow
- fatty acid
- transcription factor
- adipose tissue
- insulin resistance
- mesenchymal stem cells
- induced apoptosis
- chronic obstructive pulmonary disease
- oxidative stress
- cell cycle arrest
- genome wide analysis
- bone mineral density
- metabolic syndrome
- type diabetes
- postmenopausal women
- gene expression
- signaling pathway
- endoplasmic reticulum stress
- cell death
- pi k akt
- big data
- machine learning
- cell proliferation
- body composition
- cystic fibrosis
- skeletal muscle
- high fat diet induced
- high density
- air pollution
- deep learning
- nitric oxide
- bone regeneration