Efficient CRISPR/Cas9 genome editing in a salmonid fish cell line using a lentivirus delivery system.

Remi L GratacapTim ReganCarola E DehlerSamuel A M MartinPierre BoudinotBertrand ColletRoss D Houston

Published in: BMC biotechnology (2020)

The optimised lentiviral-mediated CRISPR method reported here increases possibilities for efficient genome editing in salmonid cells, in particular for future applications of genome-wide CRISPR screens for disease resistance.

Keyphrases

genome editing
crispr cas
genome wide
induced apoptosis
dna methylation
cell cycle arrest
copy number
high throughput
oxidative stress
cell death