Comparison of RNA isolation methods on RNA-Seq: implications for differential expression and meta-analyses.
Amanda N ScholesJeffrey A LewisPublished in: BMC genomics (2020)
Within a self-contained experimental batch (e.g. control versus treatment), the method of RNA isolation had little effect on the ability to identify differentially expressed transcripts. However, we suggest that researchers performing meta-analyses across different experimental batches strongly consider the RNA isolation methods for each experiment.