Systematic evaluation of C. elegans lincRNAs with CRISPR knockout mutants.

Shuai WeiHe ChenEmmanuel Enoch DzakahBin YuXiaolin WangTao FuJingxin LiLei LiuShucheng FangWeihong LiuGe Shan

Published in: Genome biology (2019)

By using CRISPR/cas9 technology, we generate knockout strains of 155 C. elegans lincRNAs as valuable resources for studies in noncoding RNAs, and we provide biological insights for 23 lincRNAs with the phenotypes identified in this study.

Keyphrases

crispr cas
genome editing
escherichia coli
wild type
genome wide
gene expression
dna methylation
case control