Use of NanoBiT and NanoBRET to monitor fluorescent VEGF-A binding kinetics to VEGFR2/NRP1 heteromeric complexes in living cells.

Chloe J Peach Laura E Kilpatrick Jeanette Woolard Stephen J Hill

Published in: British journal of pharmacology (2021)

This NanoBiT approach enabled real-time ligand binding to be quantified in living cells at 37°C from a specified complex between a receptor TK and its co-receptor for the first time.

Keyphrases

living cells
fluorescent probe
single molecule
vascular endothelial growth factor
binding protein
endothelial cells
aqueous solution