Electroacupuncture Pretreatment Exhibits Lung Protective and Anti-Inflammation Effects in Lipopolysaccharide-Induced Acute Lung Injury via SIRT1-Dependent Pathways.
Dan LuoLi LiuHai-Ming ZhangYu-Dian ZhouMin-Feng ZhouJin-Xiao LiZhao-Min YuQian TangShu-Rui YangRui ChenFeng-Xia LiangPublished in: Evidence-based complementary and alternative medicine : eCAM (2022)
To investigate the effect of electroacupuncture (EA) on acute lung injury (ALI), a lipopolysaccharide (LPS) induced ALI mouse model was used in this study. Before receiving intratracheal LPS instillation, mice were given EA at ST36 for 7 days as a long-term treatment or one time as a short-term treatment. Lung histopathological examination, lung injury scores, lung wet/dry (W/D) ratio, and inflammatory cytokines included proinflammation factors such as TNF- α , IL-1 β , and IL-6 and anti-inflammation factors such as IL-4 and IL-10 in serum and bronchoalveolar lavage fluid (BALF) were detected at the end of experiment. The results show that EA pretreatment ameliorated the lung damage and inflammatory response by LPS. In addition, we found that SIRT1 and its deacetylation of NF- κ B were promoted after EA pretreatment in lung tissues. Meanwhile, the expression of angiotensin-converting enzyme 2 (ACE2) is also enhanced by EA pretreatment. Thus, the present findings suggest that EA could be a potential therapy of ALI.
Keyphrases
- inflammatory response
- lps induced
- lipopolysaccharide induced
- oxidative stress
- toll like receptor
- angiotensin converting enzyme
- mouse model
- angiotensin ii
- poor prognosis
- neuropathic pain
- rheumatoid arthritis
- ischemia reperfusion injury
- mesenchymal stem cells
- nuclear factor
- stem cells
- combination therapy
- metabolic syndrome
- blood brain barrier
- signaling pathway
- cerebral ischemia
- climate change
- cell therapy