Targeted base editing in rice and tomato using a CRISPR-Cas9 cytidine deaminase fusion.

Zenpei ShimataniSachiko KashojiyaMariko TakayamaRie TeradaTakayuki ArazoeHisaki IshiiHiroshi TeramuraTsuyoshi YamamotoHiroki KomatsuKenji MiuraHiroshi EzuraKeiji NishidaTohru AriizumiAkihiko Kondo

Published in: Nature biotechnology (2017)

We applied a fusion of CRISPR-Cas9 and activation-induced cytidine deaminase (Target-AID) for point mutagenesis at genomic regions specified by single guide RNAs (sgRNAs) in two crop plants. In rice, we induced multiple herbicide-resistance point mutations by multiplexed editing using herbicide selection, while in tomato we generated marker-free plants with homozygous heritable DNA substitutions, demonstrating the feasibility of base editing for crop improvement.

Keyphrases

crispr cas
genome editing
high glucose
diabetic rats
climate change
gene expression
cancer therapy
dna methylation