Characterization of Green Fluorescent Protein in Heart Valves of a Transgenic Swine Model for Partial Heart Transplant Research.
Katherine BisharaJennie H KwonMorgan A HillKristi L HelkeRussell A NorrisKristin WhitworthRandall S PratherTaufiek Konrad RajabPublished in: Journal of cardiovascular development and disease (2023)
A transgenic strain of pigs was created to express green fluorescent protein (GFP) ubiquitously using a pCAGG promoter. Here, we characterize GFP expression in the semilunar valves and great arteries of GFP-transgenic (GFP-Tg) pigs. Immunofluorescence was performed to visualize and quantify GFP expression and colocalization with nuclear staining. GFP expression was confirmed in both the semilunar valves and great arteries of GFP-Tg pigs compared to wild-type tissues (aorta, p = 0.0002; pulmonary artery, p = 0.0005; aortic valve; and pulmonic valve, p < 0.0001). The quantification of GFP expression in cardiac tissue allows this strain of GFP-Tg pigs to be used for future research in partial heart transplantation.
Keyphrases
- aortic valve
- poor prognosis
- pulmonary artery
- transcatheter aortic valve replacement
- aortic valve replacement
- transcatheter aortic valve implantation
- aortic stenosis
- coronary artery
- pulmonary hypertension
- heart failure
- gene expression
- pulmonary arterial hypertension
- quantum dots
- atrial fibrillation
- coronary artery disease