Community-developed checklists for publishing images and image analyses.
Christopher SchmiedMichael S NelsonSergiy V AvilovGert-Jan BakkerCristina BertocchiJohanna BischofUlrike BoehmJan BrocherMariana T CarvalhoCatalin ChiritescuJana ChristopherBeth A CiminiEduardo Conde-SousaMichael EbnerRupert C EckerKevin W EliceiriJulia Fernandez-RodriguezNathalie GaudreaultLaurent GelmanDavid GrünwaldTingting GuNadia HalidiMathias HammerMatthew HartleyMarie HeldFlorian JugVarun KapoorAyse Aslihan KoksoyJudith LacosteSylvia E Le DévédecSylvie Le GuyaderPenghuan LiuGabriel G MartinsAastha MathurKota MiuraPaula Montero LlopisRoland NitschkeAlison NorthAdam C ParslowAlex L Payne-DwyerLaure PlantardRizwan AliBritta Schroth-DiezLucas SchützRyan T ScottArne SeitzOlaf SelchowVed P SharmaMartin SpitalerSathya SrinivasanCaterina Strambio-De-CastillaDouglas J TaatjesChristian TischerHelena Klara JamborPublished in: Nature methods (2023)
Images document scientific discoveries and are prevalent in modern biomedical research. Microscopy imaging in particular is currently undergoing rapid technological advancements. However, for scientists wishing to publish obtained images and image-analysis results, there are currently no unified guidelines for best practices. Consequently, microscopy images and image data in publications may be unclear or difficult to interpret. Here, we present community-developed checklists for preparing light microscopy images and describing image analyses for publications. These checklists offer authors, readers and publishers key recommendations for image formatting and annotation, color selection, data availability and reporting image-analysis workflows. The goal of our guidelines is to increase the clarity and reproducibility of image figures and thereby to heighten the quality and explanatory power of microscopy data.
Keyphrases
- deep learning
- optical coherence tomography
- high resolution
- convolutional neural network
- single molecule
- artificial intelligence
- high speed
- healthcare
- big data
- high throughput
- electronic health record
- clinical practice
- mental health
- label free
- primary care
- mass spectrometry
- data analysis
- quality improvement
- fluorescence imaging
- loop mediated isothermal amplification