In vitro anti-breast cancer study of hybrid cinnamic acid derivatives bearing 2-thiohydantoin moiety.
Dalal Nasser BinjawharFawziah A Al-SalmiMaha Ali AlghamdiOla A Abu AliEman FayadYoustina William RizzkNourhan M AliIbrahim Mohey El-DeenElsayed H EltamanyPublished in: Future medicinal chemistry (2024)
Aim : To synthesize new hybrid cinnamic acids ( 10a , 10b and 11 ) and ester derivatives ( 7 , 8 and 9 ) and investigate their anti-breast cancer activities. Materials & methods: Compounds 7-11 were evaluated ( in vitro ) for their cytotoxic activities against the MCF-7 cell line. A flow cytometry examination was performed. Protein levels of nuclear factor erythroid 2-related factor 2 (Nrf2), topoisomerase II and caspase-9 were measured by qRT-PCR. Molecular docking studies were conducted. Results : Several components were discovered to be active, mainly component 11 , which induced arrest in the cell cycle at phase S, greatly decreased the expression of Nrf2 and topoisomerase II; and upregulated the expression of caspase-9. Conclusion: The newly thiohydantoin-cinnamic acid hybrids can contribute to creating promising candidates for cancer drugs.
Keyphrases
- cell cycle
- molecular docking
- nuclear factor
- flow cytometry
- poor prognosis
- cell proliferation
- oxidative stress
- cell death
- toll like receptor
- binding protein
- molecular dynamics simulations
- induced apoptosis
- papillary thyroid
- diabetic rats
- squamous cell
- long non coding rna
- breast cancer cells
- signaling pathway
- childhood cancer
- endoplasmic reticulum stress
- immune response