Integrative analysis of bulk and single-cell RNA sequencing reveals the gene expression profile and the critical signaling pathways of type II CPAM.
Fengxia LiZheng TanHongyu ChenYue GaoJie XiaTing HuangLiang LiangJian ZhangXianghong ZhangXucong ShiQiang ChenQiang ShuLan YuPublished in: Cell & bioscience (2024)
In summary, by integrating bulk RNA-seq data of type II CPAM with scRNA-seq data, the gene expression profile and critical signaling pathways such as GPCR signaling and PI3K-AKT signaling pathways were revealed. Abnormally expressed genes in these pathways may disrupt epithelial-mesenchymal transition and contribute to the development of CPAM. Given the effectiveness of prenatal treatments of microcystic CPAM using maternal steroids and maternal betamethasone administration, targeting the genes and signaling pathways involved in the development of CPAM presents a promising therapeutic strategy.
Keyphrases
- single cell
- signaling pathway
- rna seq
- pi k akt
- epithelial mesenchymal transition
- genome wide
- gene expression
- high throughput
- induced apoptosis
- cell cycle arrest
- genome wide identification
- electronic health record
- cell proliferation
- big data
- transforming growth factor
- randomized controlled trial
- systematic review
- birth weight
- pregnant women
- pregnancy outcomes
- genome wide analysis
- preterm birth
- cancer therapy
- machine learning
- cell death
- deep learning
- copy number
- data analysis
- drug delivery
- body mass index
- transcription factor
- network analysis
- weight gain