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CRISPR/Cas9-mediated мultiplexed multi-allelic mutagenesis of genes located on A, B and R subgenomes of hexaploid triticale.

Dmitry N MiroshnichenkoVadim TimerbaevMikhail DivashukAlexander PushinValeria AlekseevaPavel KroupinMikhail BazhenovMariya SamarinaAleksey ErmolaevGennady KarlovSergey Dolgov
Published in: Plant cell reports (2024)
The first-time generation of hexaploid triticale plants harbouring variable panels of novel mutations in gene families involved in starch biosynthesis has been achieved by the subgenome-independent multiplexed CRISPR/Cas9-mediated editing.
Keyphrases
  • crispr cas
  • genome editing
  • genome wide
  • genome wide identification
  • single cell
  • copy number
  • dna methylation
  • cell wall
  • genome wide analysis