Effects of Extracts from Tiaozhi Granule and Its Components on Expression of Scavenger Receptor Class B Type I.
Xiao YuXiao-Dong ZhaoRong-Qi BaoJia-Yu YuGuo-Xing ZhangJing-Wei ChenPublished in: Evidence-based complementary and alternative medicine : eCAM (2016)
Sera from the rats with different drug treatments (atorvastatin, Tiaozhi granule, or its extracts) were collected. LO-2 cells or HepG2 cells were pretreated with different sera as the following groups randomly: (1) blank control group, (2) positive control group (atorvastatin group), (3) Tiaozhi granule water extract groups, (4) Tiaozhi granule alcohol extract groups, and (5) alcohol extracts for each component: Pollen Typhae Angustifoliae, Curcuma longa L., and Rhizoma Alismatis. LO-2 cells were cotransfected with plasmid carrying SR-BI and pRL-TK promoter genes. Promoter activity was measured by the luciferase reporter gene assay. The mRNA and protein expressions of SR-BI were examined using real-time PCR and western blot analyses. Our results show that promoter activity and mRNA and protein expression levels of the SR-BI were significantly upregulated by Tiaozhi granules alcohol or water extracts in a dose-dependent manner. Pollen Typhae Angustifoliae alcohol extract with a high dosage could also increase SR-BI activity and expression, but not the extracts from Curcuma longa L. and Rhizoma Alismatis. Both Tiaozhi granule alcohol and water extracts can upregulate SR-BI gene expression. Among the components, Pollen Typhae Angustifoliae are important for the regulatory effect coordinating with Curcuma longa L. and Rhizoma Alismatis.
Keyphrases
- gene expression
- dna methylation
- induced apoptosis
- binding protein
- alcohol consumption
- transcription factor
- oxidative stress
- poor prognosis
- genome wide
- cell cycle arrest
- real time pcr
- anti inflammatory
- emergency department
- cell proliferation
- small molecule
- long non coding rna
- pi k akt
- electronic health record
- genome wide analysis
- amino acid