Prediction of human iron bioavailability using rapid c-ELISAs for human plasma hepcidin.

The biological variability in plasma hepcidin, (inter-sample CV) was 5-10-fold higher for both the Peninsula and DRG assay than the analytical variably (inter-run within-sample CV) suggesting substantial discriminatory power to distinguish biological hepcidin variation. Between methods, prediction of iron bioavailability in generally healthy iron depleted subjects appears comparable.