Newcastle Disease Virus Virotherapy: Unveiling Oncolytic Efficacy and Immunomodulation.
Kawther A ZaherJehan S AlrahimiFatemah S BasingabAlia M AldahlawiPublished in: Biomedicines (2024)
Cell viability was low in HCT116 and HT-29, indicating a cytotoxic effect in the MTT assay. NDV+TCL recorded the highest rate of cell death (56.72%). NDV+TCL had accelerated cell death after 48 h, reaching 58.4%. The flow cytometry analysis of the blood and tumor of mice with induced tumor treated with combined treatment revealed elevated levels of CD83, CD86, CD8, and CD4 (76.3, 66.9, 83.7, and 14.4%, respectively). The ELISA levels of IFN-γ, IL-4, and IL-12 in serum and the spleen homogenate were elevated (107.6 ± 9.25 pg/mL). In contrast, the expression of IL-10 was significantly reduced (1 ± 0.79).
Keyphrases
- cell death
- disease virus
- flow cytometry
- cell cycle arrest
- poor prognosis
- magnetic resonance
- immune response
- high glucose
- type diabetes
- high throughput
- nk cells
- high fat diet induced
- oxidative stress
- signaling pathway
- adipose tissue
- insulin resistance
- long non coding rna
- skeletal muscle
- endothelial cells
- pi k akt
- stress induced
- anti inflammatory
- wild type