A Simple Protocol for Sample Preparation for Scanning Electron Microscopic Imaging Allows Quick Screening of Nanomaterials Adhering to Cell Surface.
Anca Emanuela MinutiLuminita LabuscaDaniel Dumitru HereaGeorge StoianHoria ChiriacNicoleta LupuPublished in: International journal of molecular sciences (2022)
Preparing biological specimens for scanning electron microscopy (SEM) can be difficult to implement, as it requires specialized equipment and materials as well as the training of dedicated personnel. Moreover, the procedure often results in damage to the samples to be analyzed. This work presents a protocol for the preparation of biological samples to evaluate the adherence of nanomaterials on the cell surface using SEM. To this end, we used silicon wafers as a substrate to grow cells and replaced difficult steps such as the critical point drying of the samples in order to make the method quicker and easier to perform. The new protocol was tested using two different types of cells, i.e., human osteosarcoma cells and adipose-derived mesenchymal stem cells, and it proved that it can grossly preserve cell integrity in order to be used to estimate nanomaterials' interaction with cell surfaces.
Keyphrases
- electron microscopy
- cell surface
- induced apoptosis
- cell cycle arrest
- randomized controlled trial
- high resolution
- single cell
- endothelial cells
- cell therapy
- type diabetes
- cystic fibrosis
- stem cells
- cell death
- escherichia coli
- staphylococcus aureus
- skeletal muscle
- pseudomonas aeruginosa
- photodynamic therapy
- bone marrow
- liquid chromatography
- simultaneous determination