Systematic optimization of Cas12a base editors in wheat and maize using the ITER platform.
Christophe GaillochetAlexandra Peña FernándezVera GoossensKatelijn D'HalluinAndrzej DrozdzeckiMyriam ShafieJulie Van DuyseGert Van IsterdaelCamila GonzalezMattias VermeerschJonas De SaegerWard DeveltereDominique AudenaertDavid De VleesschauwerFrank MeulewaeterThomas B JacobsPublished in: Genome biology (2023)
Our data show that ITER is a sensitive, versatile, and high-throughput platform that can be harnessed to accelerate the development of genome editing technologies in plants. We use ITER to create an efficient Cas12a-ABE by iteratively testing a large panel of vector components. ITER will likely be useful to create and optimize genome editing reagents in a wide range of plant species.