Login / Signup

ITR-Seq, a next-generation sequencing assay, identifies genome-wide DNA editing sites in vivo following adeno-associated viral vector-mediated genome editing.

Camilo BretonPeter M ClarkLili WangJenny A GreigJames M Wilson
Published in: BMC genomics (2020)
In sum, ITR-Seq is a powerful method for identifying off-target sequences induced by AAV vector-delivered genome-editing nucleases. ITR-Seq will help us understand the specificity and efficacy of different genome-editing nucleases in animal models and clinical studies. This information can help enhance the safety profile of gene-editing therapies.
Keyphrases
  • genome editing
  • genome wide
  • crispr cas
  • copy number
  • dna methylation
  • circulating tumor
  • gene therapy
  • rna seq
  • sars cov
  • single cell
  • high throughput
  • single molecule
  • cell free
  • health information
  • nucleic acid