Restored in vivo-like membrane lipidomics positively influence in vitro features of cultured mesenchymal stromal/stem cells derived from human placenta.
Alexandros ChatgilialogluMartina RossiFrancesco AlvianoPaola PoggiChiara ZanniniCosetta MarchionniFrancesca RicciPier Luigi TazzariValentina TaglioliPhilip C CalderLaura BonsiPublished in: Stem cell research & therapy (2017)
Culturing hFM-MSCs alters their fatty acid composition. A tailored lipid supplement is able to improve in vitro hFM-MSC functional properties by recreating a membrane environment more similar to the physiological counterpart. This approach should be considered in cell therapy applications in order to maintain a higher cell quality during in vitro passaging and to influence the outcome of cell-based therapeutic approaches when cells are administered to patients.
Keyphrases
- cell therapy
- stem cells
- mesenchymal stem cells
- fatty acid
- endothelial cells
- end stage renal disease
- bone marrow
- single cell
- chronic kidney disease
- newly diagnosed
- ejection fraction
- induced apoptosis
- prognostic factors
- peritoneal dialysis
- cell cycle arrest
- umbilical cord
- patient reported outcomes
- oxidative stress
- induced pluripotent stem cells
- cell proliferation
- endoplasmic reticulum stress